The Rainbow Cruiser appears in Rainbow Ride, where Mario can get to it by using a series of Magic Carpets. He first has to take the Magic Carpet to the right of the course's starting point, then ride the Magic Carpet to the right of the spinning platforms, and lastly the Magic Carpet to the left at the end of the second carpet's path. Once at the Rainbow Cruiser, the player must jump over platforms and Falling Blocks to climb onto it.
On the Rainbow Cruiser, there is a heavy wind that blows Mario to the stern. Two Bob-ombs and a Lakitu are on the deck, and a cannon is on the stern. The Power Star for the first mission, Cruiser Crossing the Rainbow, is on the ship's bow. A 1-UP Mushroom can be found on the tip of the bowsprit. The ship's mast is in the center, and a 1-UP Mushroom spawns from it when climbed to the top. Once the cannon is opened, by talking to the Bob-omb Buddy at the start of Rainbow Ride, Mario can use it to reach a platform with a Chuckya and a yellow block containing the Power Star for the sixth mission, Somewhere Over the Rainbow.
The Rainbow Cruiser has some differences in the remake. One of the Bob-ombs is replaced with a Goomba. The Star and 1-UP Mushroom have switched spots, with the Star on the very end of the bowsprit and the 1-UP Mushroom simply on the bow. A red block containing a Power Flower was added near the stern.
Slip Slidin' Away • Lil' Penguin Lost • Big Penguin Race • Frosty Slide for 8 Red Coins • Snowman's Lost His Head • Wall Kicks Will Work (N64)/Mario's Super Wall Kick (DS) • Switch Star of Cool, Cool Mountain (DS)
Big Boo's Haunt
Go on a Ghost Hunt • Ride Big Boo's Merry Go Round • Secret of the Haunted Books • Seek the 8 Red Coins • Big Boo's Balcony • Eye to Eye in the Secret Room • Switch Star of the Basement
Hazy Maze Cave
Swimming Beast in the Cavern • Elevate for 8 Red Coins • Metal-Head Mario Can Move! (N64)/Metal-Head Wario Can Move (DS) • Navigating the Toxic Maze • A-Maze-Ing Emergency Exit • Watch for Rolling Rocks • Underground Switch Star
In the Talons of the Big Bird • Shining Atop the Pyramid • Inside the Ancient Pyramid • Stand Tall On the Four Pillars • Free Flying For 8 Red Coins (5th Star, N64) (7th Star, DS) • Tox Box Switch Star (DS) • Pyramid Puzzle
Snowman's Big Head • Chill with the Bully • In the Deep Freeze (N64) • Yoshi's Ice Sculpture (DS) • Whirl from the Freezing Pond • Shell Shreddin' for Red Coins (N64) • Snowman's Silver Star (DS) • Into the Igloo • Red Coins in the House (DS)
Wet-Dry World
Shocking Arrow Lifts! • Top o' the Town • Secrets in the Shallows and Sky (N64)/5 Secrets in the Shallows and Sky (DS) • Express Elevator--Hurry Up! • Go to Town for Red Coins (N64)/Go to Town for the Red Coins (DS) • Quick Race Through Downtown! • Soaked Silver Stars (DS)
Tall, Tall Mountain
Scale the Mountain • Mystery of the Monkey Cage • Scary 'Shrooms, Red Coins • Mysterious Mountainside • Breathtaking View from Bridge (Star 5, N64)/Breathtaking View from the Bridge (Star 7, DS) • Blast to the Lonely Mushroom (Star 6, N64) (Star 5, DS) • 5 Secrets of the Mountain
Roll into the Cage (N64) • Luigi in the Cage (DS) • The Pit and the Pendulums (N64) • The Pendulum Switch Star (DS) • Get a Hand • Stomp on the Thwomp •Timed Jumps on Moving Bars • Stop Time for Red Coins • Tick Tock Silver Stars
Rainbow Ride
Cruiser Crossing the Rainbow • The Big House in the Sky • Coins Amassed in a Maze • Swingin' in the Breeze • Tricky Triangles! • Somewhere over the Rainbow • Switch Star of the Manor