Queen B. is the third boss in Donkey Kong Country resembling a giant Zinger. Queen B. is found in Vine Valley. Queen B. tries to attack Donkey and Diddy with her massive stinger. Like regular Zingers, Queen B. cannot be jumped on due to the spikes on her back. The Kongs must attack Queen B. with a barrel, making her angry and red and bounces across the entire area. The Kongs cannot attack Queen B. in this state, due to being invincible in this mode. After a while, Queen B. calms down and turns yellow again. She can also have a barrel be thrown at her again. The Kongs must repeat these step four more times to defeat Queen B. and move on to Gorilla Glacier.
In the Game Boy Advance remake, Queen B. has regular Zingers surrounding her instead of bouncing up and down multiple times by herself an having to wait a certain amount of time to defeat Queen B. When all of the Zingers surrounding Queen B. become defeated, Donkey and Diddy can attack Queen B. with a barrel.
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| Characters | Playable characters | Donkey Kong • Diddy Kong • Cranky Kong • Funky Kong • Candy Kong • Rambi the Rhino • Enguarde the Swordfish • Winky the Frog • Squawks the Parrot • Expresso the Ostrich |
| Bosses | Very Gnawty • Master Necky • Queen B. • Really Gnawty • Dumb Drum • Master Necky Snr. • King K. Rool | |
| Enemies | Enemies | Army • Bitesize • Chomps • Chomps Jr. • Clambo • Croctopus • Gnawty • Klaptrap • Klump • Krash • Kritter • Krusha • Manky Kong • Mincer • Mini-Necky • Necky • Rockkroc • Slippa • Squidge • Zinger |
| Objects | Objects | Animal Token • Banana • Barrel • Extra Life Balloon • K-O-N-G Letters • Mine Cart • Tire |
| Worlds and Locations | Worlds and Locations | Donkey Kong Island • Kongo Jungle • Monkey Mines • Vine Valley • Gorilla Glacier • Kremkroc Industries Inc. • Chimp Caverns • Donkey Kong's Treehouse • Cranky's Cabin • Funky's Flights • Candy's Save Point • Gangplank Galleon |
| Levels | Levels | Jungle Hijinxs • Ropey Rampage • Reptile Rumble • Coral Capers • Barrel Cannon Canyon Winky's Walkway • Mine Cart Carnage • Bouncy Bonanza • Stop & Go Station • Millstone Mayhem Vulture Culture • Tree Top Town • Forest Frenzy • Temple Tempest • Orang-utan Gang • Clam City Snow Barrel Blast • Slipslide Ride • Ice Age Alley • Croctopus Chase • Torchlight Trouble • Rope Bridge Rumble Oil Drum Alley • Trick Track Trek • Elevator Antics • Poison Pond • Mine Cart Madness • Blackout Basement Tanked Up Trouble • Manic Mincers • Misty Mine • Necky Nutmare (GBC only) • Loopy Lights • Platform Perils |