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Penny's Lab is a location that first appears in WarioWare Gold. It is a laboratory where Penny does her research, and is the setting for her story in the Twist League.
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| Characters | Main characters | 5-Volt • 9-Volt • 18-Volt • Ana • Ashley & Red • Dr. Crygor • Dribble & Spitz • Jimmy T • Mona • Orbulon • Penny • Wario (Wario Deluxe) • Young Cricket & Master Mantis | |
| Minor characters | 13-Amp • Alien Bunny • Amy and Mimi • Ben • Cat • Danny • Dark Lord Hum Gree • Doris 1 • Fronk • Gahrumble • Joe • Little Hammy • Lulu • Mike• Mr. Sparkles • Ms. Munchly • Ninja Steve • Pops • Ralphie • Ruffington • Sasquatch Family • Space Gangs | ||
| Microgames | Mash League | Wario | Busted! • Crazy Cars • Eye Spy • Loot Scoot • No Solicitors • Super Wario Bros. • Wario Whirled
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| Jimmy T. | 100 Meter Dash • And the Winner is... • Ballroom Basics • Big Drip • Body Block • City Surfer • Close Call • Diddly Squat • Extreme Patty-Cake • Getcha Groove On • Goal in One • Hand Me Down • Heads Up • High Hoops • Hurry Hurdles • Judo Pro • Jumpin' Rope • Lift & Shout • Off-Fence • Punch Out • Rugby
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| Mona | The Brush Off • Butterfingers • Catterwall • Cavity Calamity • Chicken Pinch • Dry Eye • Eating Out • Electric Groove Fish • Fit for a Frame • Fries With That? • Gold Digger • I Came, I Saw • Kitty Cover • Mole • Nighttime Allergies • Nail Call • Noodle Cup • Paddle Parade • Parking Lot Problems • Right of Way • Sloppy Salon • Stalled Out • Tip the Scale • What's the Toadal? • Worm Squirm
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| Dribble & Spitz | Arrow Space • Classic Clash • Code Buster • Cover Up • Crack Down • Dungeon Dilemma • Find My Behind • The Frog Flap • Gravitational Pull • Ground Control • Hover for Cover • Labyrinth • Long Lost Love • Manic Mechanic • Mars Jars • Munch a Bunch • Nose Dive • Picture Perfect • RAAAARRRRGGGHH • Rocky Reunion • Stop! Go! • Strength in Numbers • Word Up
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| 5-Volt | Balloon Fight • Donkey Kong Country • Fire Emblem Awakening • Game & Watch: Manhole • Ice Climber • Joker Face • Kid Icarus • Lefty RX • The Legend of Zelda • Mario Bros. • Mario Clash • Ocarina of Time • One Hit Wonder • Pinball • Pushmo • Rhythm Tweezers • Super Mario Kart • Super Mario Land • Yoshi's Island
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| Twist League | Wario | Cut to the Chase • Focus Pocus • Force Feeding • High Treeson • Pumping Iron • Road Wario • Universal Marionette
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| Ashley & Red | A Moment of Reflection • A-maze-ing • Bacon Patrol • Crescent Roll • Crossing Guard • Doggy Door • Foiled Again! • Make Way for Martian • Manual Labor • Monster Chase • Net Profits • Pipe Puzzle • Power Outage • Road Work • Safecracker • Shave the World • Space Passage • Stab in the Dark • Stumblebot • Tearful Reunion • Walk This Way
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| Dr. Crygor | Armed and Dangerous • Barrel Roll • Basic Training • Curls and Weigh • Dodge Ball • Fancy Footwork • Feel the Burn • Heavy Lifting • Iron Stomach • Micro Golf • Ride the Wave • Self-Defense • Shoot the Fruit • Slam Dunk • Snowboard Slalom • Sync or Sink • Top Off • Wheel of Whack • Wiggle Room
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| 18-Volt | Animal Crossing: Wild World • Chiritorie • Donkey Kong Jr. • Dr. Mario • Laser Pointer • Metroid • Metroid-Samus • NES Open Tournament Golf • Pikmin 2 • Racing 112 • R.O.B. Block Set • Star Fox • Super Mario Bros. • Super Mario Bros. 3 • Super Mario Sunshine • Super Mario World • Wario Land 3 • Wii Menu • The Wind Waker
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| Penny | Balancing Act • Cheeky Monkey • Clean Sweep • Code Dependency • County Fair • Cutting It Close • Fashion Plate • Fine Tune • Fragile! • Get to the Point • Half Empty • Home Haircut • Kit-tease • Peak-a-boo-boo • Picky Eater • Round It Out • Sew Easy • Strike a Chord • Toilet Training • Tooth Haste • Tooth Trouble • What Goes In... • Wrecking All
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| Touch League | Wario | Into the Sunset • Loose Change • One-Liner • Scratch and Match • Steel Chef • Thanks a Latte • Balloon Brigade | |
| Kat & Ana | Bug Eyed • Burning Sensation • Cheap Thrill • Clog It • Coin Count • Crowded Desktop • Going Up • Good Times • Impressionism • Off-line • Oh, the Irony • On the Mark • Oral Hijinks • Page Panic • Right in the Eye • Salad Daze • Secret Ingredient • Sleepy Head • Sticking Point • Top Notch • Tune Out • Up for Grabs • Very Attractive • Write On, Dude • XYZ!
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| 9-Volt | amiibo Tap • Clawing for More • Donkey Kong • Duck Hunt • Excitebike • Hanafuda • Head Count • Joy-Con Slide • Love Tester • Mario Paint - Flyswatter • Mii Channel • Nintendogs • Phantom Hourglass • Sounds of Super Mario Bros. • Stylus Hunt • Super Mario Maker • Ten Billion Barrel • Tomodachi Life • Wrecking Crew
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| Young Cricket | Arms Race • Count 'Em Up • Engine Trouble • Erosion • Finish Line • Flag Waver • Heat Wave • I'm Gonna Hurl • Local News • Long Division • Oh, Snap! • Pass Aggressive • Pass Fail • Pit Stop • Pro Bowling • Pumped Poser • Rump Roast • Skate Race • Stroke of Genius • Sweeps Week • Twin Swimmers
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| Orbulon | Big Bang • Birdcicle • Breaking Up • Character Development • Crypto Zoo • Family Reunion • Fruit Fans • Goin' Loopy • Hookin' Up • Love Struck • On the Edge • Over the Hump • Pet Peeved • Plant Food • The Proud, the Fuse • Separation Anxiety • Sinking Feeling • Snack Sucker • Sneaky Snatcher • Sneeze, Please! • Spirit Spot • Stripy Sea • Sure, You Can
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| Ultra League | Answering Machine • The Claw • Feed on Fronk • Fronk Fishing • Sliding Doors • Tread Carefully • Vicious Veggies | ||
| Wario Deluxe | |||
| Leagues | Leagues | Mash League • Touch League • Twist League • Ultra League (Dancing League • Potluck Gang) • Wario Deluxe | |
| Challenge | Modes | All Mixed Up • Battle Time • Cruise Controls • Sneaky Gamer • Split Screen • Super Hard • Thrill Ride • Wario Interrupts • Wario Watch | |
| Locations | Locations | 5-Volt's House • Agate Forest • Ashley's Mansion • Club Joe • Crystal Park • Diamond City • Diamond City Amusement Park • Diamond City Castle • Diamond City Elementary • Diamond City Stadium • Dr. Crygor's Lab • Emerald Street • Gigantaburger • Joe's Clothes • Luxeville • Penny's Lab • Peridot Campgrounds • Sapphire Street • The Farthest Edge of Space • Wario's House | |
| Objects | Objects | Computer • Dribble Taxi • Hydrocannon LX • Mandrake • Octoblazooka • Oinker • Pot of Luxeville • Super-Special Tummy Tonic • Tonally Transformative Elixir • Wario Bowl | |
| Miscellaneous | Other | Gallery | |