The player must tap the blue heart panel for Peach to activate Gloom. While using Gloom, Princess Peach can grow plants, spin water wheels, run faster, and jump higher. Gloom is also required to beat bosses: Petey Piranha and Gooper Blooper.
King Boo used Gloom after Peach depleted three lives from him. His tears inflict damage to Peach, forcing the player to control her to go above him. King Boo will also attempt to extinguish the lamps with his tears.
Giant Kamek used Gloom after Peach depleted three lives from him. His clothing changes from violet to blue. As a result, the cloud disappears forcing Peach to rise above him. Giant Kamek also appears either on the left side or the right side of the screen.