A Big Ant Trooper is larger than a normal Ant Trooper.
Ant Trooper appears as an enemy. Ant Troopers made their appearance in Ant Trooper Hill as the titular enemy. The player can jump on then without either of them being harmed. In fact, jumping on them is mandatory to get through Ant Trooper Hill. They are invincible and cannot be destroyed at all, even with a Super Star.