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Anti-gravity is a new feature of Mario Kart 8 and used again in Mario Kart 8 Deluxe. It has the character's wheels flat on the surface, on the view of the course, the character will be looking upside down or sideways. Its areas have special blue panels that have circles to mark their borders.
Courses for the Nitro Grand Prix
- Mario Kart Stadium - Mario Kart Stadium used the anti-gravity for the course after turning a straight angle until gliding off the ramp.
- Water Park - After turning the right, the anti-gravity section until after turning a straight angle.
- Sweet Sweet Canyon - After the underwater segment.
- Thwomp Ruins - Near the end of the course, players will get put on a slightly inclined segment that ends in a Glider section.
- Mario Circuit - This course has anti-gravity for all but the right-side up sections, due to the course's mobius-strip shape.
- Toad Harbour - There is an optional section at the hairpin.
- Twisted Mansion - Just after entering the mansion, racers come across an anti-gravity section on the walls. They later come across an underwater segment and race on the walls.
- Shy Guy Falls - After the first two turns.
- Sunshine Airport - Between the two glider sections near the end of the course.
- Dolphin Shoals - At the big clockwise loop.
- The Electrodrome - After the first Glider section and two turns, the course becomes anti-gravity. This ends at another glider section.
- Mount Wario - At the start of the second section and along the Wario Dam. The third section also has a big downwards slope that is in anti-gravity.
- Cloudtop Cruise - After the first glider section, racers will race upside-down inside the thundercloud.
- Bone-Dry Dunes - There is a section near the Glider section that has players race on a wall of bones.
- Bowser Castle - Throughout the first section. The Bowser Golem also appears at the second one, punching it. It then goes down after a right turn. Another right turn leads to the Glider section.
- Rainbow Road - After the first glider section.
- Dragon Driftway - All but the starting section.
- Mute City - Entire level.
- Ice Ice Outpost - Before the glider section that leads to the start of the area.
- Hyrule Circuit - Inside Hyrule Castle.
- Wild Woods - Entire first section.
- Big Blue - Entire level.
- GBA Mario Circuit - The hairpin turn has been inclined by the Ultra Hand.
- N64 Toad's Turnpike - Players can now race on the walls at the first 180 degree turn and the last turn.
- 3DS DK Jungle - The golden temple's interior has been tilted 30 degrees to the east, requiring anti-gravity.
- DS Wario Stadium - The two turns that have the firebars now have been suspended just like in GBA Mario Circuit.
- GCN Sherbet Land - Falling into the lake near the start reveals an anti-gravity section.
- 3DS Piranha Plant Slide - The underwater section has been tilted 30 to 45 degrees to the west.
- Wii Wario's Gold Mine - The mine section has been made to an anti-gravity section. Collision with the minecarts no longer causes damage, but instead provides a boost.
- GCN Baby Park - Entire level.
- GBA Ribbon Road - Entire section post the second jump. This ends in a glider section that replaces the long straightaway.
- 3DS Neo Bowser City - Final section.
- GBA Cheese Land - Entire section after the first jump. This one engulfs an entire glider section and leads to the starting area.