It is nighttime at the Mushroom Kingdom. Everyone seems to be sleeping well...except for Toad. As he tosses and turns, he starts having a nightmare. In his nightmare, he sees Toadette caught in the talons of Wingo, and Wingo is with Bowser and Tatanga. Tatanga smacks Toad and says to him, "You're pathetic, little mushroom head! Did that master Mushroom head of yours pick you up from an orphanage or something?" before laughing at him and calling him a doofus. Toad screams as he sees Bowser claw Toadette in the face, killing her. As Toad wakes up, he starts hyperventilating, only to find that Toadette is in bed, right next to him. At Bowser's castle, Tatanga is speaking to Bowser about a plan to wipe out Toad and his friends, but to keep Toadette alive. The next morning, Peach sees that Toad is very tired and scared. Toadsworth is so worried about Toad that he calls upon Rosalina for his assistance. Rosalina agrees, and they all head off to Bowser's castle. But Toad is still worried about something bad happening to Toadette. He even states that he was wrong to feel brave when fighting Wingo. As they reach Bowser's castle, they are very disappointed to see no one inside. Mario wonders where they could have gone. Then, Toad sees a UFO in the sky. That's when they realize Bowser's plan with Tatanga. Luckily, Mario finds a giant spaceship (It's big enough for all of them to fit in). As they head into space, they see a strange planet they've never seen before. Mario wonders what it is. As they put on their spacesuits, Mario asks where they are. Tatanga explains that they're on his home planet, and that he teamed up with Bowser back in 2009. Toad is horrified to see that Tatanga has his very own laboratory. Just then, another giant spaceship appears. This one, however, was piloted by Bowser Jr., who brought many Goombas, Koopas (Including the Koopalings), Bob-Ombs, and Petey Piranha. Tatanga is very pleased, as he was expecting a great big battle between them and team Mario. He officially calls the enemy team "Team Tatanga". As we expect, there is a great big battle. Bowser Jr. proves to be very cunning, and since the Goombas are in great numbers, our heroes are easily overpowered. Tatanga then sees Toadette, ties her up, gags her with duct tape and takes her to his lab. Bowser Jr. tries to guard the entrance, but Mario easily knocks him out. Then comes another brawl, on the way through the lab. As they all get through the doors to the end, they watch in horror to see Toadette put in a cage over a pit of snakes. Tatanga is surprised, but Bowser explains his true intentions, to use Toadette as bait for Toad so that he can rapidly feed him to the snakes. He wants to see the look on Toadette's face before he finishes the rest of her friends off. This starts yet another battle, with Mario, Luigi, Yoshi and Toad against Bowser and Peach, Daisy, Rosalina and Birdo against Tatanga. As they are fighting, Toad escapes the battle to free Toadette. He takes the duct tape off her mouth and opens the cage. She smiles, but then Bowser jumps up and because Toad avoids the killing blow, Bowser instead breaks the chains securing the cage, and needless to say, Toadette falls into the pit of snakes. Peach throws a rope, and Toadette struggles to climb as the snakes keep biting her. As she finally makes it to the top, she falls face-first onto the ground. Toad runs to her, and to his disbelief, she is covered in snake bites. Just then, a Bob-Omb explodes and sets off destruction in the lab, much to Tatanga's horror. Bowser shoves him away and furiously blames Toad for what happened to Toadette. They leave just before the lab collapses completely. As they get back to Earth, Toad apologizes to Peach for his overconfidence. Peach simply says that Toad was very brave and very lucky. Daisy hopes that Rosalina can use her wand to heal Toadette, but Rosalina isn't so sure.
The end, with Toadette saying her "S's" like a rattlesnake.